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KMID : 1094720120170040812
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 4 p.812 ~ p.817
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Controlling chitosan’s molecular weight via multistage deacetylation
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Yaghobi N.
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Abstract
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Chitosan samples with different molecular weights (Mw) and degree of deacetylation (DD) were prepared by controlling operating conditions throughout the multistage alkali treatment. The temperature of the reaction, time duration and number of reaction steps were considered effective parameters. A database was developed for chitosan preparation in order to achieve high degrees of deacetylation and control the molecular weight of chitosan without changing other molecular structures. The number of treatments and the duration of each step of deacetylation significantly affected molecular weight so that two samples were obtained with a DD of 99% and two different molecular weights ranging from 4.66×105 to 2.93×105 Based on these results, the highest molecular weight obtained using the multistage treatment without decreasing DD was 5.32×105, with a DD of 96.67%. Also, the morphological studies indicate that the molecular weight of chitosan has a significant effect on the pore size of the prepared scaffolds. However, this effect is critical. In other words, the pore size will increase by increasing molecular weight of chitosan from low upto medium molecular weight and when it reached to high molecular weight the pore size is decreased.
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KEYWORD
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biopolymer, chitosan, molecular weight, degree of deacetylation, intrinsic viscosity
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